International Conferences And Symposiums

Advances in Knowledge Acquisition: 9th European Knowledge by Richard Benjamins, Christine Pierret-Golbreich (auth.),

By Richard Benjamins, Christine Pierret-Golbreich (auth.), Nigel Shadbolt, Kieron O'Hara, Guus Schreiber (eds.)

This booklet offers the refereed court cases of the ninth ecu wisdom Acquisition Workshop, EKAW '96, held in Nottingham, united kingdom, in could 1996.
The 23 revised complete papers incorporated tackle the main appropriate theoretical and applicational points of information acquisition with a undeniable emphasis at the acquisition of information for the modelling or automation of complicated problem-solving behaviour. the quantity is geared up in sections on theoretical and common matters, eliciting wisdom from textual or different resources, data-mining, staff elicitation, and planning.

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Additional resources for Advances in Knowledge Acquisition: 9th European Knowledge Acquisition Workshop, EKAW '96 Nottingham, United Kingdom, May 14–17, 1996 Proceedings

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Furthermore, a competitive procedure was used for lactoferrin determination in which the antibody (anti-lactoferrin) was conjugated with horseradish peroxidase enzyme using a biotinylation process. 19 20 2. Methods The biotinylation of lactoferrin and the subsequently used competition procedure for the measurement by the lactoferrin inmunosensor is illustrated in figure 1. 2 step Bxtizvidinpermidose addition 3 step Competition between Lactofenin immobilizd on membrane and Lactofernin free in solutioi for anlihody coni.

D. R. Longo, F. J. Kim, Znorg. Nucl. Chem. 32, 2443 (1970). 4. S. C. C. Schreiman, Tetraherdon Lett. 27, 4969 (1986). 5 . S. C. C. Marguerettaz, J. Org. Chem. 52,827 (1987). Lashkaryov Institute of Semiconductor Physics, National Academy of Sciences, Prospect Nauki, 41, Kyiv, 03028, Ukraine MARIA GRAZIA MANERA, JOLANDA SPADAVECCHIA, PIETRO SICILIANO, ROBERTO RELLA Insituto per la Microelettronica ed i Microsistemi, via perMonteroni, Lecce, 73100, Italy We propose a novel technique to detect DNA hybridization.

Among these DNA biosensors appears promising in order to detect the eventually occurring binding between DNA and BaP and/or its by-products (ox)BaP. The aim of this experimentation was the evaluation of graphite screen printed electrodes (SPE) for the detection of BaP-DNA adducts formation. Chronopotentiometry was used as detection strategy to monitor the adduct formation. To achieve this goal, the specific aims of this experimentation were: i) to develop an experimental procedure to detect guanine oxidation electrochemical signal, ii) to develop an experimental protocol to oxidize BaP to produce reactive species able to form DNA adducts, iii) to evaluate the BaPDNA adduct formation.

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